fbpx

Blog

News, Tutorials, Tips and Tricks
Evaluation of Eukaryotic Gene Finding with Augustus in OmicsBox

Evaluation of Eukaryotic Gene Finding with Augustus in OmicsBox

  The OmicsBox Genome Analysis Module allows executing eukaryotic de-novo and RNA-seq based gene finding with Augustus. In this way, it is possible to discover novel, putative coding genes and their genomic positions for yet uncharacterized genome. Based on the Augustus algorithm an ‘ab-initio’ (DNA sequences only), as well as RNA-seq guided (BAM files) gene predictions, are supported. We will

Read More »

Pairwise Differential Expression Analysis

The Pairwise Differential Expression Analysis tool is designed to perform differential expression analysis of count data arising from an RNA-seq experiment. The application, which is based on the software package “edgeR”, allows the identification of differentially expressed genes between two experimental conditions by applying quantitative statistical methods. This video shows the performance of a pairwise differential expression analysis in which

Read More »

Time Course Expression Analysis

A simple use-case comparing OmicsBox with R chunks for Time Course Expression Analysis The Blast2GO feature “Time Course Expression Analysis” is designed to perform time-course expression analysis of count data arising from RNA-seq technology. Based on the software package ‘maSigPro’, which belongs to the Bioconductor project, this tool allows the detection of genomic features with significant temporal expression changes and

Read More »

Pairwise Differential Expression Analysis

A simple use-case comparing OmicsBox with R chunks The OmicsBox feature “Pairwise Differential Expression Analysis” is designed to perform differential expression analysis of count data arising from RNA-seq technology. This tool allows the identification of differential expressed genes considering two different conditions based on the software package ‘edgeR’, which belongs to the Bioconductor project. This use case shows the basic

Read More »

How to translate longest ORFs

The Blast2GO “Translate Longest ORF” tool searches for the longest Open Reading Frame (ORF) in nucleotide sequences and translates them into their protein sequences. You may choose one or multiple of the six possible DNA frames or select the reading frame based on the frame of the best blastx hit. In this video, we will explain how to translate a set of

Read More »
New Blast2GO Version 3.3

Major Release Version 4

(Release Date: 13/09/2016) We are very happy to announce the release of Blast2GO 4. This new version contains many new bioinformatic features like Differential Expression Analysis, Gene Finding or Gene Set Enrichment Analysis. Blast2GO will update automatically if activated. Otherwise please download the latest version online from here. Feedback, questions, as well as feature requests, are most welcome. Please write

Read More »

Functional Analysis of Pancreatic Cancer Expression Profiles

This use case shows how to perform a functional analysis of pancreatic cancer expression data with Blast2GO. The performed steps are explained more in detail with short video tutorial linked to each section. Malignant (PDAC), benign (CP) and normal tissue (NP) gene lists are compared against each other. The human genome functional annotation data is retrieved via BioMart and used

Read More »

How to perform a Gene Set Enrichment Analysis (GSEA)

This article explains what a Gene Set Enrichment Analysis (GSEA) is, how it works and how to use it with OmicsBox. What is an enrichment analysis? Enrichment analyses are a family of bioinformatics methods that aim to facilitate the biological interpretation of many bioinformatics results. Among these, the methods based on gene sets analysis are particularly helpful and widespread. The

Read More »

How to use PSORTb

This tutorial shows how to assign subcellular localizations with PSORTb in OmicsBox/Blast2GO. We also explain how to merge the obtained results to the existing functional annotations of a Blast2GO project. OmicsBox/Blast2GO allows to assigns sub-cellular localization sites to proteins based on their amino acid sequence via PSORTb. PSORTb is an algorithm which can be applied to bacteria or archaea protein sequences

Read More »
Search

Blog Categories:

News

Releases, Media, Announcements, etc.

Tips And Tricks

Mini-tutorials for common use-cases and to address frequently asked questions FAQs

Video Tutorials

Helpful Features, Tips and Tricks

Use Cases, Reviews, Tutorials

Product Tutorial, Quickstarts, New Features, etc.

Most Popular: