Introduction A common preprocessing step in metagenomics data analysis e.g. working with host-associated studies is to remove the host-related DNA from the sequencing data. This is often referred to as contamination removal. A common approach to isolate host-related reads is performing a read mapping against the host genome. Bowtie2 is the reference tool for this task. Since OmicsBox 2.0 this
Introduction The IsoSeq sequencing method produces full-length transcripts using Single Molecule, Real-Time (SMRT) Sequencing. Long read lengths allow sequencing of full-length transcripts up to 10 kb or longer, removing the need for transcript assembly or inferencing. Therefore, unlike traditional short-read methods, IsoSeq provides a more accurate and complete view of gene expression and alternative splicing events. This comprehensive understanding of
Introduction Given a new genome, one of the first and most important tasks is determining the structure of its protein-coding genes. Ab initio gene prediction algorithms play a critical role because they produce gene structures quickly, inexpensively, and remarkably reliable. In OmicsBox, the Eukaryotic Gene Finding application is based on AUGUSTUS, which is one of the most accurate programs for
Introduction Single-cell RNA sequencing (scRNA-seq) is a technology that aims to study the expression profiles of individual cells, in contrast with the typical bulk RNA sequencing that analyses the expression of large populations of cells together. scRNA-seq allows the identification of different cell types within the same sample, so it is possible to study tissues with more detail. One of
Introduction A pathway analysis is an important step in the final biological interpretation of experimental results in most omics studies. For non-model species however this often becomes a difficult endeavor due to the lack of functional information. The Combined Pathway Analysis tool allows to identify pathways from multiple pathway databases for any set of sequences. In combination with differential expression
BioBam announced today the release of OmicsBox 2.0. This new version of BioBam’s bioinformatics solution offers a user-friendly data analysis platform which allows to get from next generation sequencing reads to biological insights with ease. The software offers “out-of-the-box” data analysis and functional interpretation of genome, transcriptome and metagenome datasets for industry, academic and governmental research biologists. OmicsBox is used
With OmicsBox version 2.0 we introduce a new login dialog. This login is required for a more advanced subscription usage management. The login credentials are identical with the ones used for the BioBam Account website. If you do not yet have an BioBam Account you can easily create one from within OmicsBox via “SIGN UP”. If you forgot your password
This project shows how OmicsBox was used to functional annotate Moringa oleifera and to better understand the genome of the plant.
Activate development on Blast2GO was discontinued in 2018. All its functionality and more new features are now part of OmicsBox. Because Blast2GO is no longer updated, some of its functions may have stopped working because web services have changed or security certificates have expired, among other things. It is highly recommended to use OmicsBox instead of Blast2GO to benefit from
