Tips, Tricks and Video Tutorial

Mini-tutorials for common use-cases and to address frequently asked questions FAQs

IsoSeq long-read analysis

IsoSeq De-Novo Isoform Discovery with OmicsBox

Introduction The IsoSeq sequencing method produces full-length transcripts using Single Molecule, Real-Time (SMRT) Sequencing. Long read lengths allow sequencing of full-length transcripts up to 10 kb or longer, removing the need for transcript assembly or inferencing. Therefore, unlike traditional short-read methods, IsoSeq provides a more accurate and complete view of gene expression and alternative splicing events. This comprehensive understanding of

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Gene Prediction Augustus OmicsBox

Eukaryotic Gene Finding with OmicsBox

Introduction Given a new genome, one of the first and most important tasks is determining the structure of its protein-coding genes. Ab initio gene prediction algorithms play a critical role because they produce gene structures quickly, inexpensively, and remarkably reliable. In OmicsBox, the Eukaryotic Gene Finding application is based on AUGUSTUS, which is one of the most accurate programs for

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Single Cell Clustering Seurat

Single Cell Clustering With Seurat in OmicsBox

Introduction Single-cell RNA sequencing (scRNA-seq) is a technology that aims to study the expression profiles of individual cells, in contrast with the typical bulk RNA sequencing that analyses the expression of large populations of cells together. scRNA-seq allows the identification of different cell types within the same sample, so it is possible to study tissues with more detail. One of

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Combined Pathway Analysis with OmicsBox

Introduction A pathway analysis is an important step in the final biological interpretation of experimental results in most omics studies. For non-model species however this often becomes a difficult endeavor due to the lack of functional information. The Combined Pathway Analysis tool allows to identify pathways from multiple pathway databases for any set of sequences. In combination with differential expression

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New OmicsBox Login Dialog

With OmicsBox version 2.0 we introduce a new login dialog. This login is required for a more advanced subscription usage management. The login credentials are identical with the ones used for the BioBam Account website. If you do not yet have an BioBam Account you can easily create one from within OmicsBox via “SIGN UP”. If you forgot your password

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InterProScan with Blast2GO: How to make it work again

Activate development on Blast2GO was discontinued in 2018. All its functionality and more new features are now part of OmicsBox. Because Blast2GO is no longer updated, some of its functions may have stopped working because web services have changed or security certificates have expired, among other things. It is highly recommended to use OmicsBox instead of Blast2GO to benefit from

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Cloud Blast and Cloud Units: How to use resources wisely

Using cloud resources efficiently is crucial for researchers dealing with large datasets. This article will show you how to use CloudBlast effectively, helping you save on computational costs and avoid common pitfalls. OmicsBox allows you to perform NCBI Blast searches using a cloud system for high-performance computing. This system runs jobs in parallel and autoscales depending on demand. To control

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How to run Blast of the differential expressed genes?

It is possible to run Blast only on the differential expressed genes and not on all the data with OmicsBox. One has to select only those sequences that have differential expressed genes in the OmicsBox project.First,  make sure that the name of the sequences in the project match the ones from the differential expression results.

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